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Non Surgical Hair Regrowth

People who desire a non-surgical regrowth strategy that is safe and effective choose the Regenerative Hair Regrowth Procedure. This revolutionary procedure combines Micro-channeling PRP and nutrients injected and your own stem cells into the scalp to encourage hair growth.

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Adult Adipose Derived Stem Cell

Our facility is the first dedicated full time hair restoration facility in the Central Florida region to offer stem cell therapy from cells that are derived from the fat cells of our adult patients. From the Stromal Vascular Fraction (SVF) of the extraction process, these stem cells are rich in mesenchymal stem cells and growth factors. Dr Rahman, offers our hair loss patients the most sophisticated FDA approved stem cell device for obtaining adult stem cells from the fat cells of patients.

We specialize in hair loss and provide surgical and non-surgical treatment to men and women who suffer from hair loss. In addition to providing non-surgical adult derived stem cells (ADSC), we also advocate proven medical therapies such as Compounded minoxidil, DHT blockers, Low Level Laser Light Therapy (LLLT), Platelet Rich Plasma (PRP) and oral supplements.

Adult Adipose Derived Stem Cell

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What is Regenerative Hair Growth?

People who desire a non-surgical regrowth strategy that is safe and effective choose the Regenerative Hair Regrowth Procedure. This revolutionary procedure combines Micro-channeling PRP and nutrients injected and your own stem cells into the scalp to encourage hair growth.

In hair restoration, Adult Adipose derived stem cells is combined with PRP (platelet rich plasma) and injected into the scalp. This application causes miniaturized hair follicles to become healthier and larger, producing more robust hair growth. Adult stem cells activates follicular progenitor angiogenesis and localized cell growth. Effective for both male and female pattern hair loss.

Regenerative Hair Growth
Regenerative Hair Growth

How does Regenerative Hair Growth Work?

The Regenerative Hair Regrowth treatment takes about 90 minutes to complete. First, a blood sample is obtained which is specially processed to produce the PRP. A local anesthetic is then administered to completely numb the scalp treatment area. The PRP and nutrients are combined and then administered via a series of injections over the full.

The recovery from the procedure is typically uneventful. Pain is easily managed in most cases with non-narcotic analgesic medication such as Ibuprofen. Some mild swelling of the forehead is occasionally seen 1-2 days after injections. This is normal and will resolve in 48 hours. Avoiding vigorous activity is recommended until the swelling is gone. There is no other downtime or noticeable effects of the procedure. Patients can wash their hair normally immediately after the injections.

New hair growth can be seen as early as 2 months, but is typically evident between 4-8 months and will continue to improve up to a year after treatment. To date, results have been maintained without additional injections for up 2 years in many patients, but no long term data is yet available to conclude that induced hair growth is permanent.

What types of conditions respond to the Regenerative Hair Regrowth treatment?

The Regenerative Hair Regrowth technique is a versatile tool in the treatment of hair loss for a number of causes including:

  • Male Pattern Hair Loss – especially in the early stages of balding, or anytime there is a lot of miniaturized hair on the scalp
  • Female Pattern Hair Loss – at all stages of hair loss
  • Hypotrichosis (fine/reduced hairs) of the eyebrows or beard
  • Alopecia Areata, Alopecia Totalis
  • Metabolic causes of hair loss – iron deficiency and medication induced hair loss

As not all patients and their hair loss condition are the same, it is important to consult with our physician if you are interested in commencing the ideal treatment program for hair growth that is right for you.

Regenerative Hair Growth

The Latest Advance in Hair Regeneration Therapy Using Proteins Secreted by Adipose-Derived Stem Cells

Adipose-derived stem cells (ADSCs) are one of the latest scientific breakthroughs in the eld of aesthetic surgery. For many years, doctors have discarded the fat tissue removed during cosmetic surgeries, such as tummy tucks, liposuction, and breast reduction. However, it was recently found that adult stem cells can be isolated from the fat tissues and then used to rejuvenate hair and skin tissues.

Most of the research concerning mesenchymal stem cells (MSCs) has focused on bone marrow mesenchymal stem cells (BMSCs). However, it is now known that MSCs exist in normal adipose tissue. ADSCs are easily isolated from fat tissue harvest by liposuction, and they can differentiate into several types of lineages in culture. ADSCs are likely to be pluripotent MSCs, which share similar characteristics with BMSCs. ADSCs have paracrine effects on their surrounding environment through the secretion of many growth factors, such as vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), basic broblast growth factor (bFGF), platelet-derived growth factor (PDGF), keratinocyte growth factor (KGF), transforming growth factor (TGF)-E1, insulin-like growth factor-binding protein (IGF-BP) precursors, bronectin, and superoxide dismutase (SOD).

These proteins secreted by ADSCs exert diverse skin-rejuvenating effects, such as the stimulation of collagen synthesis and broblast migration during wound healing. In addition, ADSC-conditioned medium (ADSC-CM) inhibits melanogenesis in B16 melanoma cells and produces skin-whitening effects. ADSC-derived secretory factors also protect dermal broblasts from oxidative stress induced by chemical and ultraviolet B irradiation. Interestingly, our improved method for harvesting ADSC-CM under hypoxic conditions yields ADSC-CM that appears to promote hair growth.Because conventional treatments for skin aging induce new collagen synthesis via the activation of dermal broblasts, we decided to consider ADSCs as a possible tool in antiaging skin therapy. Several studies have found hair growth-stimulating effects of ADSC growth factors in ex vivo animal models; therefore, we performed a clinical study of ADSC-CM for human hair regeneration. We used a trichogram to obtain preliminary data that hair counts in certain areas increased with treatment frequency.

Most studies of ADSC-based therapies have been in vitro animal model studies with satisfactory results. However, there are safety issues with respect to the direct application of cultured ADSCs to human skin; in particular, this technique might create a risk of cancer. In addition to safety concerns, it is currently very difcult to commercialize ADSCs. Therefore, new approaches are needed to overcome the limitations of cellular therapy. Fortunately, the proteins secreted from ADSCs might have great advantages over cell-based therapy for skin regeneration. Because of its long-term stability, lyophilized ADSC-CM avoids most of the safety issues associated with cell-based therapies. The use of ADSC-CM may also enhance the scalability of production, which would allow for the development of low-cost therapeutics.

A commercial advanced adipose-derived stem cell protein extract (AAPE) was developed by a Korean research team at Prostemics Co, Ltd (Seoul, Korea). This extract contains numerous growth factors and regeneration-promoting proteins. Its main components include PDGF (44.41 ± 2.56 pg/mL), bFGF (131.35 ± 30.31 pg/mL), KGF (86.28 ± 20.33 pg/mL), TGF-E1 (103.33 ± 1.70 pg/mL), HGF (670.94 ± 86.92 pg/mL), VEGF (809.53 ± 95.98 pg/mL), collagen (921.47 ± 49.65 pg/mL), bronectin (1466.48 ± 460.21 pg/mL), and SOD in 5 mL saline solution. In the present study, we examined the ability of AAPE injections combined with buƀomedyl (0.15E0.25 mg), vitamin B1 (5 mg), vitamin B6(2.5E5.0 mg), vitamin H (1 mg), vitamin C (80E100 mg), vitamin E (5 mg), and coenzyme Q10 (10 U), along with amino acid nappage mesotherapy, to improve the function of mesotherapy with antioxidant effects and induce hair growth. We refer to this combined therapy as hair regenerative therapy (HARG) enhanced by hypoxic ADSC-CM.

Materials and Methods

Adipose tissue was voluntarily donated by 5 healthy 20-year-old women and was examined for any possible bacterial and viral infections. After the adipose tissue was collected during elective liposuction, ADSCs isolated from the aspirates were cultured under hypoxic conditions. Finally, the ADSC-CM was concentrated by centrifugation and freeze-dried as a commercial AAPE (Prostemics Co, Ltd). This product was examined for possible bacterial and viral infections, such as Staphylococcus aureus, Streptococcus pneumonia, Streptococcus pyogenes, Escherichia coli, Salmonella typhimurium, Proteus mirabilis, Enterococcus faecalis, Bacillus subtilis, Bacteroides vulgates, Candida albicans, Clostridium sporogenes, Mycoplasma arthitidis, Mycoplasma orale, Mycoplasma pulmonis, Mycoplasma hyorhinis, Mycoplasma salivarium, Mycoplasma fermentans, Mycoplasma pneumoniae, Mycoplasma bovis, Acholeplasma laidlawii, human immunodefciency virus, hepatitis B virus, and hepatitis C virus. AAPE was manufactured in accordance with the guidelines of Korean Food and Drug Administration for cell therapy. Informed consent for using AAPE addressed safety and possible risks such as infections.

before and after

For the hair regeneration treatments, vitamin B, buƀomedyl, cysteine, vitamin H, vitamin C, vitamin E, and coenzyme Q10 were added to AAPE. Buƀomedyl and AAPE are not FDA approved by the US Food and Drug Administration. Mesotherapy techniques such as nappage and papule injections with 30G or 32G needles were used; however, the point-by-point technique was not used. Nappage involved sticking the skin every 3 mm to penetrate the clear zone of skin (without bleeding) with a 30G needle attached to a solution-lled syringe. Papule injections provided 0.02 to 0.05 mL of AAPE per square centimeter in each intradermal injection for a total volume of 3E4 mL per treatment. Patients received 4 treatment sessions every 3E5 weeks until hair regeneration was observed. The patients received follow-up examinations at 2- to 4-month intervals for at least 1 year after the nal treatment session.

Regenerative Hair Growth


The effect of hair regenerative therapy was evaluated in 25 patients (Table): 12 men with androgenic alopecia, 1 man with androgenic alopecia and alopecia areata, and 12 women with female pattern androgenic alopecia (including 3 women with diffuse alopecia). Finasteride was administered to 5 male patients during the study: 3 had a history of fnasteride use before the study and continued the medication, 2 men started finasteride during the study, and 1 man stopped nasteride medication when the study started. No female patients took nasteride or any supplementary medication. These patients agreed to provide self-assessments of treatment satisfaction using a visual analog scale (VAS), which is an arbitrary point scale ranging from 1 (dissatisfaction) to 5 (satisfaction) (Figure 1). Patient self-evaluations were performed after each of the first 4 sessions. Patients evaluated their satisfaction with every treatment 3 to 4 weeks after the previous treatment. Physiciandetermined scores were assessed for 25 patients after the fourth treatment with the following VAS: 1 = worse, 2 = no change, 3 = slight improvement, 4 = improvement, and 5 = excellent improvement. Physician-determined assessment and patientsk satisfaction with the fourth treatment were determined 4E6 weeks after the fourth treatment. Differences with P < .01 were taken as statically signifcant.


Protein solution from ADSC-CM applied during 4 treatment sessions within 3E4 months induced considerable increases in hair growth. Interestingly, protein solution from ASDC-CM promoted hair growth at the frontal region of the head in patients with androgenic alopecia (Figures 2 through 6). Positive responses regarding treatment were obtained from all patients in the present study. We had previously studied only 1 patient with iatrogenic alopecia and several patients with alopecia areata; however, these patients are not included with the present 25 patients because we do not have the VAS score sheets from the earlier patients. We compared HARG therapy and a meso-cocktail with mesotherapy using a side-by-side comparison (Figure 7). There were no adverse effects, including bacterial infection and granuloma. Hair regrowth was maintained for at least 1.5 years, but this was dependent on patient age. Men older than 60 years had the least maintenance of hair regrowth, followed by men 50E60 years old and women older than 60 years (Figure 6). Men younger than 50 years and women younger than 60 years maintained hair regrowth between 1.5 and 2.5 years.

Friedmanks 2-way analysis of variance and Wilcoxonks signed rank test were used to establish statistical signicance in the self-assessments of treatment satisfaction performed by 25 patients. Statistical significance was set at P< .01. The analyses were performed with commercially available statistical software (Excel, Microsoft, Redmond, Wash; Statcel3 Excel, OMS Publishing Inc, Tokorozawa, Japan).

The mean patient-determined VAS scores were 2.52 after the rst session, 2.92 after the second session, 3.40 after the third session, and 3.72 after the fourth session. The mean VAS scores for men were 2.46 after the first session, 2.85 after the second session, 3.38 after the third session, and 3.77 after the fourth session. The mean VAS scores for women were 2.58 after the rst session, 3.00 after the second session, 3.42 after the third session, and 3.67 after the fourth session. The VAS scores increased as the number of treatments increased. The mean patient-determined VAS score after each treatment is plotted in Figure 8. The number of patients who reported a VAS score t3 increased as the number of treatments increased.

The physician-determined score was assessed after the fourth treatment, and the mean scores were 4.20 for all patients, 4.15 for men, and 4.25 for women (Figure 10). Statistically signicant differences were determined by Friedmanks 2-way analysis of

before and after

variance (P< .01) and Wilcoxonks signed rank test (P< .01). Statistically signicant improvements in VAS scores were observed from the first treatment (P< .01). There were statistically significant differences between the physician-determined assessment scores and patient satisfaction scores

before and after


Human adipose tissues that contain ADSCs are easily obtained by suction-assisted lipectomy. Because of their pluripotent nature, stem cells may provide therapies for many symptoms and diseases. The clinical potential of MSCs has been illustrated with tissue engineering studies, and MSCs are sometimes regarded as a substantial therapy for clinical cases of some diseases. For example, in an intracranial hemorrhage mouse model, transplantation of MSCs reduced acute cerebral inƀammation and chronic brain degeneration and promoted long-term functional recovery. In a model of hepatic failure, conditioned medium from MSCs provided trophic support to the injured tissue by inhibiting cell death and stimulating regeneration. These effects have also been observed in cosmetic applications.

ADSCs have potent antioxidant activity and protect human dermal broblasts (HDFs) from oxidative injury by inhibiting apoptosis. ADSCs are good candidates for the control and prevention of skin damage from free radicals in various skin conditions. ADSCs were found to be superior to HDFs in promoting HDF proliferation and upregulating type I collagen secretion. ADSCs secretory factors protect dermal broblasts from oxidative

stress induced by chemical and ultraviolet B irradiation. When combined with traditional treatment modalities, stem cells appear to have dramatic antiwrinkle, skin-whitening, and skin-tightening effects, mediated by signicant increases in collagen synthesis and dermal thickness, improvements in the inƀammatory reactions of blood vessels, and inhibition of melanin synthesis.

Granulation after mesotherapy for alopecia has previously been reported. Granulomas may form after injection of some growth factors. The doses of growth factors in AAPE are within the physiological range. We consider that HARG therapy causes no granuloma formation because the promoting factors are colocalized with the inhibitory factors.

Growth factors are important in hair follicle development. PDGF isoforms induce and maintain the anagen phase in murine hair follicles and promote the hair stem cells to hair mother cells. In addition, VEGz controls hair growth and follicle size by angiogenesis in a VEGF transgenic mouse model. HGF alters cyclic hair growth and stimulates hair follicle growth, and IGF upregulates hair follicle growth by stimulating the proliferation of follicle cells through the signaling pathways of its receptors. IGF-BP modulates the actions of IGF-I in hair regeneration. KGF-2 signicantly stimulates human hair follicle cells.

Patient satisfaction was lower with mesotherapy treatment than with HARG therapy. Some patients moved from mesotherapy treatment to HARG therapy with stem cell protein. The stem cell proteins are the most essential components of this hair regrowth therapy. Treatment using only the vitamin mixture was not more effective than the stem cell protein treatment in our recent double-blind test. However, we expected that the addition of vitamins would be beneficial for hair growth effects such as antioxidant effects.

HARG therapy is a great alternative for hair rejuvenation in patients who are unwilling or unsuitable to undergo traditional hair rejuvenation surgery (eg, many women are inappropriate candidates for surgical hair transplantation). Thin and short hair show prominent changes in length and thickness after therapy. As patients feel changes in their hair after the first and second treatments, their satisfaction scores are relatively high. New hair appears on the head after several treatments. Patient satisfaction increases as the number of treatments increases. There was a significant difference between physician-determined assessment scores and patient satisfaction scores. This result indicates that doctors evaluate hair growth by vellus growth, whereas patients appraise hair growth by perception, such as feeling the increased density of hair. Objective and consistent means of evaluation are needed.


ADSC proteins are commercially available and do not require specialized equipment, such as a cell-processing center. These proteins can be routinely applied by a well-trained medical practitioner. We demonstrated that HARG therapy was effective for hair growth and is a potential alternative for hair regeneration in patients who are unwilling or unsuitable to undergo traditional surgical hair transplantation


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